Lipid-based Transfection Reagents Exhibit Cryo-induced Increase in Transfection Efficiency
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چکیده
منابع مشابه
Lipid-based Transfection Reagents Exhibit Cryo-induced Increase in Transfection Efficiency
The advantages of lipid-based transfection reagents have permitted their widespread use in molecular biology and gene therapy. This study outlines the effect of cryo-manipulation of a cationic lipid-based formulation, Lipofectamine 2000, which, after being frozen and thawed, showed orders of magnitude higher plasmid delivery efficiency throughout eight different cell lines, without compromising...
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BACKGROUND Fully differentiated adipocytes are considered to be refractory to introduction of siRNA via lipid-based transfection. However, large scale siRNA-based loss-of-function screening of adipocytes using either electroporation or virally-mediated transfection approaches can be prohibitively complex and expensive. METHODOLOGY/PRINCIPAL FINDINGS We present a method for introducing small i...
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Commercially available lipid-based transfection reagents are widely used to deliver DNA to cells. However, these lipid-based transfection reagents show poor gene transfer efficiency in primary cells. Here, we demonstrate a simple method to improve gene transfer efficiency in primary fibroblasts and hepatoblasts using a combination of lipid-based transfection reagents. Our data show that combine...
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The process of in vitro transfection involves introduction of genetic material into cells and it is generally used for mammalian cells and involves non-viral methods [1]. Various therapeutic cargo molecules can be used for intracellular delivery into cancer cell lines and primary cells, including plasmid DNA (pDNA), proteins, small molecules, messenger RNA (mRNA), shall RNA such as short interf...
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ژورنال
عنوان ژورنال: Molecular Therapy - Nucleic Acids
سال: 2016
ISSN: 2162-2531
DOI: 10.1038/mtna.2016.8